Thermo Fisher Scientific VASP Monoclonal Antibody (OTI2G1), TrueMAB
VASP 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot, ICC/IF, Flow cytometry에 적합. 인간 VASP full-length recombinant protein을 면역원으로 사용. 동결건조 형태, 1 mg/mL 농도, PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G1 |
| Immunogen | Full-length human recombinant protein of human VASP produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. These proteins contain an EVH1 N-terminal domain that binds proteins with E/DFPPPPXD/E motifs, targeting Ena-VASP proteins to focal adhesions.
The mid-region includes a proline-rich domain that binds SH3 and WW domain-containing proteins.
The C-terminal EVH2 domain mediates tetramerization and binds both G and F actin, contributing to filamentous actin formation and cell adhesion/motility.
VASP may also participate in intracellular signaling regulating integrin–extracellular matrix interactions and is regulated by cyclic nucleotide-dependent kinases PKA and PKG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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