Thermo Fisher Scientific NSE Monoclonal Antibody (5D3)
Human, Mouse, Rat에 반응하는 NSE 단일클론 항체로 Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. ENO2 단백질 검출에 특화되어 있으며, 신경세포 및 신경내분비세포 연구에 유용. 1 mg/mL 농도의 액상 형태로 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D3 |
| Immunogen | Purified recombinant fragment of human ENO2 (amino acids 251–433) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538543 |
Product Specific Information
MA5-17072 targets ENO2 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.
The immunogen is a purified recombinant fragment of human ENO2 (amino acids 251–433) expressed in E. coli.
MA5-17072 detects ENO2, which has a predicted molecular weight of approximately 47.3 kDa.
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis.
NSE is highly stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.
It is expressed primarily in neurons and neuroendocrine cells, and co-expression with chromogranin A is common in neuroendocrine neoplasms.
Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells.
Release of NSE from damaged neurons into CSF and blood serves as a biomarker for neuronal injury and is clinically used as a marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis.
Elevated NSE levels are also found in certain neoplasias, including pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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