Thermo Fisher Scientific HCN3 Polyclonal Antibody
Rabbit polyclonal antibody targeting HCN3 for WB, IHC, and ICC applications. Recognizes rat HCN3 C-terminal peptide (residues 727–744). Lyophilized form; reconstitute in DDW. Suitable for research on HCN channel function in heart and brain.
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- PA5111879
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide corresponding to amino acid residues 727–744 of rat HCN3 (Intracellular, C-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2857288 |
Product Specific Information
- Reconstitute antibody with 50 µL of double distilled water (DDW).
- The reconstituted solution can be stored at 4°C for up to 1 week.
- For longer storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Peptide sequence: (C)QRATGDGSPRRKGSGSER
Homology: Human and mouse amino acid residues are identical.
Target Information
HCN3 is a member of the hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channel family. HCN currents are associated with pacemaker activity in the heart and brain, control of resting potential, and neuronal plasticity. HCN3 is expressed in the brain, though at lower levels than other HCN channels.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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