Thermo Fisher Scientific SPP1 Monoclonal Antibody (1E10)
SPP1(Osteopontin) 단백질을 표적하는 마우스 모노클로날 항체로, Western blot과 ELISA에 적합. 인간 반응성, 액상 형태이며 보존제가 없음. Affinity chromatography로 정제되어 연구용으로 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 0.1 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E10 |
| Immunogen | SPP1 (NP_000573, 148 a.a.–257 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
SVVYGLRSKS KKFRRPDIQY PDATDEDITS HMESEELNGA YKAIPVAQDL NAPSDWDSRG KDSYETSQLD DQSAETHSHK QSRLYKRKAN DESNEHSDVI DSQELSKVSR
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates OPN function and integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and forming a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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