Thermo Fisher Scientific SPP1 Monoclonal Antibody (3G11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

3G11

Immunogen

SPP1 (NP_000573, 148 a.a. approximately 257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Osteopontin,uniProtId:P10451-1,ncbiNodeId:9606,antigenRange:148-257,antigenLength:314,antigenImageFileName:H00006696-M12A_Osteopontin_P10451-1_House_mouse.svg,antigenImageFileNamePDP:H00006696-M12A_Osteopontin_P10451-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: SVVYGLRSKS KKFRRPDIQY PDATDEDITS HMESEELNGA YKAIPVAQDL NAPSDWDSRG KDSYETSQLD DQSAETHSHK QSRLYKRKAN DESNEHSDVI DSQELSKVSR

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.