Thermo Fisher Scientific AES Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen, and the purity is >95% (by SDS-PAGE).

Target Information

Adhesion to the extracellular matrix regulates cell survival through integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, which are transcriptional co-repressors involved in neurogenesis, segmentation, and sex determination.
AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein released into the cytoplasm upon onset of apoptosis. This complex suppresses a survival-promoting gene transcription program controlled by the TLE protein family.
Interestingly, apoptosis of cells transfected with AES and Bit1 can be inhibited if the cells attach to fibronectin through the α5β1 integrin, suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, particularly α5β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.