Thermo Fisher Scientific YBX1 Polyclonal Antibody
YBX1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific YBX1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293–320aa ENPKPQDGKETKAADPPAENSSAPEAEQ) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2747356 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. It stabilizes cytoplasmic mRNA and modulates translation by interacting with eukaryotic initiation factors. YBX1 binds to promoters containing a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes, regulating transcription of numerous genes. It promotes separation of DNA strands containing mismatches or cisplatin modifications, exhibits endonucleolytic activity, and may contribute to DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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