Thermo Fisher Scientific E-cadherin Polyclonal Antibody
E-cadherin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 인간, 마우스, 랫 등 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체로 암 연구 및 세포 접착 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry - Free Floating (IHC (Free)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 2 publications |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E-Cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.14 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792236 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (80%), Rat (83%), Dog (80%), Pig (83%), Bovine (82%).
- Positive Control: MCF-7, HT-29.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (calcium-dependent adhesion protein) superfamily. This protein is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in the CDH1 gene (chromosome 16) are associated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-cadherin function contributes to tumor progression by enhancing proliferation, invasion, and metastasis.
E-cadherin plays a key role in tissue architecture and integrity by regulating cell growth and adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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