Thermo Fisher Scientific NEFL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:15,000

-

Immunocytochemistry (ICC/IF)

1:5,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2149935

Product Specific Information

For Western blot using alkaline phosphatase plus chromagen, a dilution of at least 1:1000 is recommended. For chemiluminescence, use at a dilution of 1:20,000.

Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.