Thermo Fisher Scientific Ub-K48 Polyclonal Antibody
Ub-K48 특이적 Rabbit Polyclonal 항체로 Western blot, ELISA, Dot blot에 적합. Human, Mouse, Rat 시료에서 반응. Affinity Chromatography로 정제된 액상 항체이며, 단백질 분해 및 유비퀴틴 경로 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| ELISA | 1 µg/mL |
| Dot Blot (DB) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence containing Human polyubiquitinated protein (K48 linkage). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.98 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914188 |
Product Specific Information
Positive test controls include: HeLa, PC-12, C2C12, Jurkat, RAW264.7, C6, Mouse heart.
Target Information
Ubiquitin exists either covalently attached to another protein or free (unanchored). When covalently bound, it forms monoubiquitin or polyubiquitin chains linked via different lysine residues (Lys-6, Lys-11, Lys-29, Lys-33, Lys-48, Lys-63) or via the initiator methionine (linear chains). Each linkage type has distinct cellular roles:
- Lys-6: DNA repair
- Lys-11: ER-associated degradation and cell-cycle regulation
- Lys-29: Lysosomal degradation
- Lys-33: Kinase modification
- Lys-48: Proteasomal degradation
- Lys-63: Endocytosis, DNA-damage response, NF-kappa-B activation
Free polyubiquitin also functions in kinase activation and signaling processes.
[Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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