Thermo Fisher Scientific Ub-K63 Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

1 µg/mL

-

Dot blot (DB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K63 linkage) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ub-K63,uniProtId:P0CG47-1,ncbiNodeId:9606,antigenRange:63,antigenLength:229,antigenImageFileName:PA5-120617_Ub-K63_P0CG47-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120617_Ub-K63_P0CG47-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914189

Product Specific Information

Positive test controls include: HeLa, HeLa+MG132, NIH/3T3, C6, Mouse brain, Rat brain.

Target Information

Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.