Thermo Fisher Scientific F(ab`)2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC
FITC로 표지된 Donkey anti-Sheep IgG (H+L) F(ab`)2 단편 항체로, 면역형광 및 유세포분석에 적합합니다. 높은 특이성과 감도를 가지며, 친화 크로마토그래피로 정제되었습니다. 498/517 nm의 여기/방출 특성을 가지며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500 – 1:20,000 |
| Flow Cytometry (Flow) | 1:500 – 1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536030 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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