Thermo Fisher Scientific F(ab`)2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 F(ab')2 Donkey anti-Sheep IgG (H+L) Secondary Antibody, AP는 양 IgG 검출용 고감도 2차 항체입니다. ELISA, WB, IHC 등 다양한 응용에 적합하며, AP 효소 결합으로 신호 증폭이 가능합니다. 친화 크로마토그래피 정제 및 안정적 저장 조건 제공.
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Thermo Fisher Scientific F(ab`)2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536032 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats Available
- Biotin conjugate
- FITC conjugate
- HRP conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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