Thermo Fisher Scientific Thymidylate Synthase Recombinant Mouse Monoclonal Antibody (rTYMS, 1884)
Recombinant mouse monoclonal antibody recognizing Thymidylate Synthase (TS). Validated for ICC/IF and Flow Cytometry. Produced in HEK293 cells, purified via Protein A/G, and supplied as a liquid at 200 µg/mL. Suitable for research on DNA synthesis and ...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rTYMS, 1884 |
| Immunogen | Recombinant human thymidylate synthase protein fragment (around aa 60–174) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), an essential step in DNA biosynthesis. TS is a critical target for fluoropyrimidines, a key class of antineoplastic drugs used in solid tumor treatment. Both 5-FU and fluorodeoxyuridine are metabolized in tumor cells to FdUMP, which inactivates TS by forming a ternary covalent complex with the folate cofactor 5,10-methylenetetrahydrofolate. TS expression levels correlate with response to 5-fluorouracil (5-FU) in colorectal, gastric, head and neck, and breast carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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