Thermo Fisher Scientific Cytokeratin Pan Type I Monoclonal Antibody (AE1)
다중 종에 반응하는 Cytokeratin Pan Type I 단클론 항체(AE1)로 WB, IHC, ICC/IF에 적합. 인간 표피 케라틴을 면역원으로 사용하며, Protein G로 정제된 액상 형태. 암 연구 및 조직 병리학적 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 12 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:125 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE1 |
| Immunogen | Human epidermal keratin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982255 |
Product Specific Information
MA5-13144 targets Cytokeratin Low Molecular Weight in WB, IF, and IHC (P) applications, showing reactivity with Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, and Rat samples.
This antibody is not recommended for NIH-3T3 cells in immunofluorescent applications.
The immunogen used is human epidermal keratin.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
Each human cytokeratin is numbered 1–20, and their expression patterns vary by epithelial type, maturation stage, and disease state.
Cytokeratin subtype expression is useful for:
- Distinguishing epithelial malignancies
- Differential diagnosis of tumors using immunohistochemistry
- Cytopathology and flow cytometry assays
Keratins are valuable markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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