Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), PE, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 33 publications

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue / Red / Violet / Yellow series (multiple options)
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436, 600, 645, 780)
• Request custom conjugation

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic domain of CD45 has tyrosine phosphatase activity, playing a key role in lymphocyte activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Recommended use at 5 µL (0.25 µg) per test in 100 µL final volume. Cell count per test: 10⁵–10⁸ cells (empirically determined).

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. Absent on non-hematopoietic cells and tissues, CD45 regulates Src-family kinases, antigen receptor signaling, and cytokine receptor pathways. Multiple isoforms arise via alternative splicing, differing in extracellular domains. CD45 is crucial in lymphocyte development, antigen signaling, and cell survival modulation through integrin-mediated pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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