Thermo Fisher Scientific HSP27 (Heat Shock Protein 27) Monoclonal Antibody (G3.1)
HSP27 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Protein A/G로 정제된 액상 형태이며, PBS buffer에 보존. 세포 스트레스 반응 및 단백질 접힘 관련 연구에 적합.
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Thermo Fisher Scientific HSP27 (Heat Shock Protein 27) Monoclonal Antibody (G3.1)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Chimpanzee, Human, Mouse, Non-human primate, Sheep, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G3.1 |
| Immunogen | Partially purified HSP27 (formerly 24K) protein from breast cancer MCF-7 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
HSPB1 (Heat Shock Protein beta-1, also known as HSP27) is a small heat shock protein that acts as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its chaperone activity, HSP27 regulates phosphorylation and axonal transport of neurofilament proteins. Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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