Thermo Fisher Scientific M-CSF Recombinant Rabbit Monoclonal Antibody (3)

Applications

• ELISA (ELISA): Tested dilution 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

This antibody has specificity for Mouse M-CSF/CSF-1.

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
M-CSF also induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
It exists in several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning protein, which can be expressed on the cell surface or further cleaved and modified in the secretory vesicle.

M-CSF is a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms modified by chondroitin sulfate addition.
Binding of M-CSF to its receptor c-Fms (CSF-1R or CD115) induces receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte–macrophage populations.

Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.