Thermo Fisher Scientific hnRNP A1 Recombinant Rabbit Monoclonal Antibody (JA39-21)
Western blot, IHC, ICC 및 Flow Cytometry에 적합한 재조합 토끼 단클론 항체. hnRNP A1 단백질을 특이적으로 인식하며 높은 특이도와 재현성을 제공. HEK293 세포에서 발현된 IgG 형식의 항체로, 단백질 A로 정제됨. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA39-21 |
| Immunogen | Synthetic peptide within Human hnRNP A1 aa 301–350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809969 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
HnRNP A1 belongs to the A/B subfamily of heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins complexed with heterogeneous nuclear RNA (hnRNA).
These proteins are associated with pre-mRNA in the nucleus and influence mRNA processing, metabolism, and transport.
HnRNP A1 contains two quasi-RRM domains that bind RNA and is one of the most abundant core proteins in hnRNP complexes, localized mainly in the nucleoplasm. It can shuttle between the nucleus and cytoplasm, likely bound to mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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