Thermo Fisher Scientific IL-4 Monoclonal Antibody (OX81), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX81

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866211

Product Specific Information

Description: This OX81 monoclonal antibody reacts with rat interleukin (IL)-4. Also known as BCDF, BCGF-1 and BSF-1, IL-4 is secreted by activated Th2 and natural killer T cells. This cytokine is also produced by Th1 and mast cells albeit to a lesser extent. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of MHC class II and CD23.

The OX81 antibody has been reported to exhibit neutralizing activity.

Applications Reported: This OX81 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting.

Applications Tested: This OX81 antibody has been tested by intracellular flow cytometric analysis of activated rat splenocytes.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.