Thermo Fisher Scientific IL-4 Monoclonal Antibody (OX81), Functional Grade, eBioscience

Thermo Fisher Scientific IL-4 Monoclonal Antibody (OX81), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats

• eFluor 660
• Request custom conjugation

Product Specific Information

This OX81 monoclonal antibody reacts with rat interleukin (IL)-4, also known as BCDF, BCGF-1, and BSF-1. IL-4 is secreted primarily by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. It promotes B cell proliferation and differentiation, and induces Ig isotype switching and upregulation of MHC class II and CD23.

The OX81 antibody has reported neutralizing activity.

Applications Reported: Intracellular staining followed by flow cytometric analysis and western blotting.
Applications Tested: Intracellular flow cytometric analysis of activated rat splenocytes.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90%, determined by SDS-PAGE.
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10%, determined by HPLC.

Target Information

Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated T cells, acting as a ligand for the IL-4 receptor. The receptor also binds IL-13, contributing to overlapping functions. STAT6 mediates IL-4 signaling. The IL-4 gene is part of a cytokine cluster on chromosome 5q, located near IL-13 and regulated by long-range elements. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.