Thermo Fisher Scientific KT3 Tag Polyclonal Antibody
KT3 태그 단백질 검출용 Rabbit Polyclonal 항체로 Western blot, ICC/IF, ELISA, IP 등에 사용 가능. KT3(KPPTPPPEPET)-KLH 면역원으로 제작되었으며, 항원 친화 크로마토그래피로 정제됨. PBS 완충액에 보관, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KT3 (KPPTPPPEPET) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with KT3 (KPPTPPPEPET) conjugated to KLH.
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
The KT3 tag does not correspond to a specific gene but is commonly used in biochemical and molecular biology research as a peptide tag for protein detection and purification.
It is related to the PI3K/AKT/mTOR signaling pathway, a key regulatory network in eukaryotic cells controlling cell survival, growth, and metabolism.
The PI3K enzyme generates phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), which acts as a second messenger facilitating Akt activation at the plasma membrane.
This pathway is frequently activated in various cancers, promoting tumorigenesis by enhancing cell growth and inhibiting apoptosis.
Targeted therapies inhibiting this pathway are under investigation for potential cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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