Thermo Fisher Scientific KT3 Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000 - 1:30,000

-

Immunocytochemistry (ICC/IF)

1:100 - 1:400

-

ELISA (ELISA)

1:1,000 - 1:30,000

-

Immunoprecipitation (IP)

1 - 4 µg/mg lysate

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KT3 (KPPTPPPEPET) conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Goats were immunized with KT3 (KPPTPPPEPET) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Target Information

The KT3 tag does not correspond to a specific gene but is often used in biochemical and molecular biology research as a peptide tag for protein detection and purification. It is related to the PI3K/AKT/mTOR signaling pathway, which is a critical regulatory network in eukaryotic cells involved in cell survival, growth, and metabolism. The PI3K (phosphatidylinositol 3-kinase) enzyme generates phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), acting as a second messenger that facilitates the translocation and activation of Akt at the plasma membrane by phosphoinositide-dependent kinases. This pathway is frequently activated in various cancers, contributing to tumorigenesis and cancer progression by promoting cell growth and inhibiting apoptosis. Targeted therapies inhibiting this pathway are being studied for their potential in cancer treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.