Thermo Fisher Scientific KT3 Tag Polyclonal Antibody
KT3 태그 인식용 염소 폴리클로날 항체로, Western blot, ICC/IF, ELISA, IP에 적합합니다. KT3(KPPTPPPEPET)-KLH 면역원으로 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 단백질 검출 및 정제 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 – 1:400 |
| ELISA | 1:1,000 – 1:30,000 |
| Immunoprecipitation (IP) | 1 – 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KT3 (KPPTPPPEPET) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with KT3 (KPPTPPPEPET) conjugated to KLH. The antibody was purified by affinity chromatography using the immobilized peptide on a solid support.
Target Information
The KT3 tag is a synthetic peptide sequence used in molecular biology for protein detection and purification. It is associated with the PI3K/AKT/mTOR signaling pathway, a key regulatory network in eukaryotic cells involved in cell survival, growth, and metabolism. Activation of this pathway is often observed in various cancers, contributing to tumorigenesis by promoting cell proliferation and inhibiting apoptosis. Research on inhibitors targeting this pathway is ongoing for potential cancer therapies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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