Thermo Fisher Scientific AU5 Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000 - 1:30,000

-

Immunocytochemistry (ICC/IF)

1:100 - 1:400

-

ELISA (ELISA)

1:1,000 - 1:30,000

-

Immunoprecipitation (IP)

1 - 4 µg/mg lysate

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AU5 (TDFYLK) conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Goats were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Target Information

AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. The recognized AU5 sequence represents the amino acid sequence TDFYLK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.