Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat, Sea urchin

Published species

Chimpanzee, Human, Mouse, Xenopus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B-5-1-2

Immunogen

Sarkosyl-resistant filament from sea urchin sperm axonemes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:Q9BQE3-1,ncbiNodeId:9606,antigenRange:1-449,antigenLength:449,antigenImageFileName:322588_alpha_Tubulin_Q9BQE3-1_House_mouse.svg,antigenImageFileNamePDP:322588_alpha_Tubulin_Q9BQE3-1_House_mouse_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532182

Product Specific Information

This antibody reacts with all forms of alpha tubulin. The expected molecular weight is ~50.5 kDa. Reactivity is confirmed with NIH3T3 cells, rat brain, mouse testis, human Caco-2, HeLa3 and PtK23 cells.

Excitation/Emission (nm) for this Alexa Fluor 488™-conjugated antibody: 495/519

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.