Thermo Fisher Scientific eIF3f Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF3f for detection of endogenous EIF3F protein in human and mouse samples. Suitable for WB and IHC applications. Purified by antigen affinity chromatography and supplied as a liquid form for research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein corresponding to residues near the C-terminal of human eukaryotic translation initiation factor 3, subunit F |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2635855 |
Product Specific Information
The antibody detects endogenous levels of total EIF3F protein.
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning for AUG recognition.
Additionally, eIF-3 is involved in disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S subunits before initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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