Thermo Fisher Scientific Human M-CSF, Animal-Free Recombinant Protein, PeproTech
인체 유래 M-CSF 단백질로 단핵구 및 대식세포 분화 연구에 적합. 동물 유래 성분이 없는 재조합 단백질. 고순도(≥98%) 및 낮은 엔도톡신(<0.1 EU/µg). 기능적 활성 검증 완료. 연구용으로만 사용 가능.
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Applications
Functional Assay (Functional)
- Assay-dependent
In vitro Assay (IV)
- Tested in assay-dependent conditions
- View 63 publications
Miscellaneous PubMed (Misc)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human, Mouse, Rat |
| Expression System | E. coli |
| Amino Acid Sequence | MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS |
| Molecular Weight | 36.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | < 0.1 EU/µg |
| Activity | ED50 ≤ 1.0 ng/ml (specific activity ≥ 1 × 10⁶ units/mg) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human M-CSF is a 36.8 kDa homodimeric protein consisting of two 159 amino acid polypeptide subunits.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, please see the lot-specific Certificate of Analysis.
Target Information
M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
It also induces VEGF secretion by macrophages, promoting endothelial progenitor cell mobilization and neovascularization.
M-CSF exists in several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning form that can be cleaved and modified in secretory vesicles.
It is a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms.
Binding to its receptor c-Fms (CSF-1R/CD115) induces receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations.
Human M-CSF shows activity on mouse cells, but mouse CSF does not act on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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