Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience
Rat IgG2a kappa 단클론 동종대조항체로, Biotin으로 결합되어 다양한 면역분석에 사용 가능. IHC, ICC/IF, Flow Cytometry 등에서 음성대조용으로 적합. Affinity chromatography로 정제되어 높은 순도 유지. 연구용으로만 사용.
- 카탈로그번호
- 13-4321-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_470083 |
Product Specific Information
Description:
The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported:
This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and ELISA.
Applications Tested:
This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostic applications. It is produced by the rat immune system and known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC).
Rat IgG2a has a molecular weight of approximately 160 kDa and consists of two heavy and two light chains. It is often used as a control or reference antibody in experiments involving rat models and is widely applied in flow cytometry and immunohistochemistry.
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Functional Grade, Pacific Orange
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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