Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), eFluor 506, eBioscience

Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), eFluor 506

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 660
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450
• FITC
• NovaFluor Series (Blue, Violet, Yellow variants)
• PE and PE derivatives (PE-Cyanine5, PE-Cyanine7, PE-eFluor 610)
• PerCP-Cyanine5.5
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR.
HLA-DR is expressed on antigen presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
It plays a critical role in peptide presentation to CD4⁺ T lymphocytes.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Fluorophore Information:

• Excitation: 405 nm
• Emission: 506 nm
• Recommended filter: 510/20 band pass
• Laser: Violet Laser

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The DRA gene comprises five exons encoding leader peptide, extracellular domains, transmembrane region, and cytoplasmic tail.
The DRB chain contains polymorphisms determining peptide binding specificity.
Hundreds of DRB1 alleles exist and are used in transplantation typing (bone marrow, kidney).
HLA-DR is expressed mainly on APCs including B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
MHC class II molecules (DR, DQ, DP) bind processed peptides and present them to T-helper cells, initiating immune responses.

HLA and MHC antibodies are essential tools in Immunopeptidomics, facilitating neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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