Thermo Fisher Scientific F(Ab`)2 Fluorescein Polyclonal Antibody, Texas Red
Goat polyclonal F(ab')₂ antibody conjugated with Texas Red for detection of fluorescein-labeled targets. Suitable for WB, ICC/IF, and ELISA. Lyophilized form with high purity and stability. For research use only.
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- 700-100-096
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| ELISA | >1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Conjugate | Texas Red |
| Excitation / Emission Max | 595 / 613 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit visible yellow-green light. This emission ceases upon removal of the excitation light. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interactions when one of the reactants is part of a cell, tissue, or other biological structure.
FITC is a commonly used marker for antibodies in immunofluorescent techniques since conjugation to proteins is relatively easy and does not generally destroy biological activity. It is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC-labeled cells by magnetic microbead-based sorting, as well as for removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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