Thermo Fisher Scientific F(Ab`)2 Fluorescein Polyclonal Antibody, Texas Red

Applications

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit visible yellow-green light. This emission ceases upon removal of the excitation light. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interactions when one of the reactants is part of a cell, tissue, or other biological structure.

FITC is a commonly used marker for antibodies in immunofluorescent techniques since conjugation to proteins is relatively easy and does not generally destroy biological activity. It is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC-labeled cells by magnetic microbead-based sorting, as well as for removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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