Thermo Fisher Scientific DNA ligase IV Polyclonal Antibody
Human DNA ligase IV에 특이적인 Rabbit Polyclonal Antibody로, IHC(Paraffin) 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 완충액에 보존. DNA 손상 복구 및 V(D)J 재조합 연구에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DNA ligase IV. Recombinant protein control fragment (Product #RP-95411) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2640648 |
Product Specific Information
- Immunogen sequence:
TYCVIAGSEN IRVKNIILSN KHDVVKPAWL LECFKTKSFV PWQPRFMIHM CPSTKEHFAR EYDCYGDSYF IDTDLNQLKE VFSGIKNSNE QTPEEMASLI ADLEYRYSWD CSPLSMFRRH TVYLDSYA - Highest antigen sequence identity to orthologs:
- Mouse: 83%
- Rat: 82%
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction.
This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ).
It forms a complex with XRCC4 and interacts with DNA-dependent protein kinase (DNA-PK), both of which are required for NHEJ.
Defects in this gene cause LIG4 syndrome.
Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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