Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
Rabbit polyclonal antibody against human ATG16L1 for WB and IHC applications. Recognizes autophagy-related protein 16L1 involved in Atg16L complex formation. Supplied lyophilized; reconstitute in sterile water. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ATG16L1 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide is homologous in mouse and rat.
- Specificity: ATG16L1.
Target Information
Autophagy related protein 16L1 is encoded by the ATG16L gene located on chromosome 2 in humans. Also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L to form an 800 kDa Atg16L complex, which facilitates LC3 lipidation to phosphatidylethanolamine (PE) — a key marker for autophagy.
The ATG16L complex, along with ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome completion.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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