Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 209 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, Biotin
• Brilliant UV/Violet series
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright series
• NovaFluor Blue/Red/Yellow variants
• Functional Grade
• Custom conjugation available

Product Specific Information

Description:
IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule via binding to hyaluronate.

Applications Reported:
Use in flow cytometric analysis.

Applications Tested:
Tested on mouse splenocytes; ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.

Fluorophore Details:
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Ensure instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation; protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in dark conditions with minimal impact on brightness.

Excitation / Emission / Laser:
633–647 nm / 780 nm / Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD44 is a 100 kDa type 1 transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells. It acts as a receptor for hyaluronic acid (HA) and mediates cell-cell interaction, adhesion, and migration. CD44 plays roles in lymphocyte activation, recirculation, and homing. Expression may increase in carcinomas and relate to metastatic potential. Multiple isoforms exist due to complex alternative splicing. Disorders associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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