Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience
CD44 단백질을 인식하는 Rat 모노클로날 항체(IM7)로, APC-eFluor 780 형광체가 결합된 제품입니다. Flow Cytometry 분석에 최적화되어 있으며, 인간과 마우스 시료에 반응합니다. 633 nm 레이저로 여기되어 780 nm에서 방출되며, 빛에 민감하므로 암조건에서 보관해야 합니다.
- 카탈로그번호
- 47-0441-8x (2개 옵션)
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience
Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Publications: View 209 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bat, Fruit fly, Human, Mouse, Non-human primate |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IM7 |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272248 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, Biotin
- Brilliant UV/Violet series
- eFluor 450, 506
- FITC
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright series
- NovaFluor Blue/Red/Yellow variants
- Functional Grade
- Custom conjugation available
Product Specific Information
Description:
IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule via binding to hyaluronate.
Applications Reported:
Use in flow cytometric analysis.
Applications Tested:
Tested on mouse splenocytes; ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Fluorophore Details:
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation; protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in dark conditions with minimal impact on brightness.
Excitation / Emission / Laser:
633–647 nm / 780 nm / Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD44 is a 100 kDa type 1 transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells. It acts as a receptor for hyaluronic acid (HA) and mediates cell-cell interaction, adhesion, and migration. CD44 plays roles in lymphocyte activation, recirculation, and homing. Expression may increase in carcinomas and relate to metastatic potential. Multiple isoforms exist due to complex alternative splicing. Disorders associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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