Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), Super Bright 600, eBioscience
인간 CD5 단백질을 인식하는 UCHT2 클론의 Mouse IgG1 단일클론 항체로, Super Bright 600 형광체가 결합된 제품입니다. Flow Cytometry 분석에 최적화되어 있으며, 비특이적 결합을 최소화하기 위한 Super Bright Staining Buffer 사용을 권장합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), Super Bright 600, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT2 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724096 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation available
Product Specific Information
The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by most thymocytes and mature T cells, and a subset of B cells. Signaling through CD5 activates T cells and mediates T-B cell interactions via CD72 binding.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Prediluted and tested on human peripheral blood cells at 5 µL (0.5 µg)/test
- Cell Range: 10⁵–10⁸ cells/test
- Excitation: 405 nm
- Emission: 600 nm
- Laser: Violet Laser
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass.
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal effect on brightness.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD5 is a 67 kDa transmembrane glycoprotein expressed on mature T-lymphocytes, most thymocytes, and certain B-cell subsets. It functions as a receptor modulating T-cell proliferation and is associated with various lymphoid malignancies and thymus cancer.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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