Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), Super Bright 600, eBioscience

Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: -

Product Specifications

Additional Formats Available:

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule.
The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on thymocytes and peripheral cytotoxic T cells.
CD8 binds to MHC class I and plays an essential role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.

Super Bright 600 is a tandem dye excited with the violet laser (405 nm) and emits at 600 nm.
Use a 610/20 bandpass filter and confirm instrument capability for this fluorochrome.

Staining Panels:
When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to reduce polymer interactions.

Light Sensitivity:
Protect from light; photo-induced oxidation may occur.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) up to 3 days at 4°C in dark with minimal impact on brightness.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD8B antigen is a cell surface glycoprotein expressed on cytotoxic T lymphocytes.
It acts as a coreceptor with the T-cell receptor to recognize antigens presented by MHC class I molecules.
The functional coreceptor can be an alpha homodimer or alpha/beta heterodimer, both showing homology to immunoglobulin variable light chains.
This gene encodes CD8 beta chain isoforms and has multiple alternatively spliced variants. A pseudogene is located on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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