Thermo Fisher Scientific Phospho-TIE2 (Tyr1108) Polyclonal Antibody
Phospho-TIE2 (Tyr1108) 폴리클로날 항체로 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Protein A로 정제된 액상 형태이며 -20°C에서 보관. 혈관 발달 및 내피세포 신호 연구에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human TIE2 around the phosphorylation site of Tyr1108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The TEK receptor tyrosine kinase (also known as TIE2) is expressed almost exclusively in endothelial cells. TEK signaling is critical for endothelial cell–smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway involved in embryonic vascular development. Immunoblotting studies have shown that TIE2 expression increases in response to thyroid-stimulating hormone and agents that elevate intracellular cAMP. Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and form a side population adhering to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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