Thermo Fisher Scientific CDH1 Monoclonal Antibody (OTI3E2), TrueMAB
Thermo Fisher Scientific의 CDH1 Monoclonal Antibody (OTI3E2)는 인간 CDH1(E-Cadherin)에 특이적인 마우스 IgG1 단클론 항체입니다. Western blot과 IHC(P)에서 검증되었으며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로 세포 접착 및 암 관련 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E2 |
| Immunogen | Full length human recombinant protein of human CDH1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin belonging to the calcium-dependent adhesion protein superfamily. It is a cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in CDH1 are associated with various cancers including gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-Cadherin function contributes to cancer progression by enhancing proliferation, invasion, and metastasis.
E-Cadherin also mediates bacterial adhesion and plays a crucial role in maintaining tissue architecture and integrity. The CDH1 gene is located on chromosome 16 in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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