Thermo Fisher Scientific Phospho-SMAD1 (Ser187) Polyclonal Antibody
Phospho-SMAD1 (Ser187) 폴리클로날 항체로, 인간·마우스·랫트 시료에서 SMAD1 인산화 단백질 검출에 적합합니다. WB, IHC, ICC, IP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 고순도(>95%) 및 높은 특이성을 제공합니다.
- 카탈로그번호
- PA536876
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- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553795 |
Product Specific Information
This antibody detects endogenous protein at a molecular weight of approximately 60 kDa.
Purity is >95% by SDS-PAGE.
Target Information
The SMAD1 protein belongs to the SMAD family, which includes proteins homologous to the Drosophila mothers against decapentaplegic (Mad) and C. elegans Sma genes. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD1 mediates bone morphogenetic protein (BMP) signaling, influencing cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand binding, SMAD1 becomes phosphorylated and forms a complex with SMAD4, regulating transcriptional activity.
SMAD1 is also a target for SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2), leading to ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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