Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PE-Cyanine5, eBioscience
마우스 Ly-6G(Gr-1) 항원을 인식하는 단클론 항체로, 중성구 특이적 결합 특성을 가짐. Flow cytometry에 최적화된 PE-Cyanine5 결합체. 0.5 µg/test 권장. 4°C 보관, 광민감성 주의. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 22 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811754 |
Additional Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, associating with B2 integrins CD11a and CD11b. The ligand for Ly-6G is currently unknown.
Unlike RB6-8C5, 1A8-Ly6g binds specifically to Ly-6G on neutrophils and has been used for neutrophil depletion with reported antagonist activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells. Recommended ≤0.5 µg/test in 100 µL volume. Cell number range: 10⁵–10⁸ cells/test. Optimize antibody titration for specific assays.
Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on signal.
Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D) is part of the myeloid differentiation antigen Gr-1 complex with Ly6C. It is a 21–25 kDa protein expressed in myeloid-derived cells of the bone marrow and serves as a marker for neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. Ly6G is implicated in anti-tumor responses and may modulate local immune responses through inhibitory signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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