Thermo Fisher Scientific EAAT3 (EAAC1) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitution: Add 50 µL or 0.2 mL of double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C. The reconstituted solution can be stored at 4°C, protected from light, for up to 1 week. For longer storage, aliquot and keep at -20°C. Avoid multiple freeze-thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

L-glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. To maintain glutamate levels below excitotoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1, and the chloride-dependent EAAT-4.
EAAC-1 is expressed in the CNS and also found in the kidney and small intestine. Structural features of glutamate transporters include eight membrane-spanning alpha-helices and a loop-pore structure unique among secondary transporters, resembling loop-pores found in ion channels. Another distinctive feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane.
Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and show reduced spontaneous locomotor activity, although no neurodegeneration was observed over 12 months.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.