
Thermo Fisher Scientific F(ab`)2-Goat anti-Human Kappa Light Chain Secondary Antibody, HRP
HRP 결합된 F(ab`)2 형태의 염소 항-인간 카파 라이트 체인 2차 항체. Western blot, IHC, ELISA 등 다양한 응용 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 민감도 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| ELISA | 1:4,000–1:8,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human kappa light chains |
| Conjugate | HRP |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.0095% Methylisothiazolone |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10985218 |
| Target | Kappa light chain |
| Antibody Form | F(ab`)2 Fragment |
Available Formats
- AP
- Biotin
- FITC
- PE
- TRITC
- Request custom conjugation available
Product Specific Information
This antibody has been cross-absorbed with pooled human myeloma proteins containing lambda light chains. Purified IgG was prepared from whole serum by affinity chromatography, and F(ab)2 fragments were generated by pepsin digestion.
Goat F(ab)2 anti-Human Kappa Light Chain antibody specifically recognizes human kappa light chains.
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets. Secondary antibodies offer flexibility across detection systems (HRP, AP, fluorescence) and enhance sensitivity via signal amplification. Typically generated by immunizing host animals with pooled immunoglobulins from the target species, these antibodies may be further purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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