Thermo Fisher Scientific SPANX Polyclonal Antibody
Human SPANX 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot 및 ELISA에 적합하며, 고순도 Affinity chromatography로 정제됨. 다양한 SPANX 패밀리 단백질과 교차 반응 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity-purified antibody prepared from rabbit serum immunized with a synthetic peptide corresponding to amino acids 25–50 of human SPANX protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | –20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at –20°C prior to opening.
- Aliquot contents and freeze at –20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only before immediate use.
This affinity-purified antibody is directed against human SPANX proteins. The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D, and SPANX-N, but not within SPANX-E.
BLAST analysis suggests cross-reactivity with SPANX proteins from gorilla and chimpanzee; reactivity with other species is unknown.
Target Information
SPANX (Sperm Protein Associated with the Nucleus on the X chromosome) family genes are testis-specific and located in a gene cluster on chromosome X. They encode proteins that localize to various subcellular compartments and play roles in spermatogenesis.
This particular SPANX gene product localizes to the nucleus and is highly expressed in metastatic cell lines, making it a potential diagnostic and prognostic marker.
As a cancer/testis antigen, SPANX represents a potential target for cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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