Thermo Fisher Scientific E6AP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length human E6-AP:GST fusion protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E6AP,uniProtId:Q05086-1,ncbiNodeId:9606,antigenRange:1-875,antigenLength:875,antigenImageFileName:PA3-843_E6AP_Q05086-1_Rabbit.svg,antigenImageFileNamePDP:PA3-843_E6AP_Q05086-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2211788

Product Specific Information

PA3-843 detects Angelman Syndrome-Associated protein (E6-AP) from human tissues.

PA3-843 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.

IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.

PA3-843 antigen is bacterially expressed, full-length human E6-AP.

Target Information

UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.