Thermo Fisher Scientific Phospho-ASK1 (Thr838) Polyclonal Antibody
Phospho-ASK1 (Thr838) 특이적 검출용 Rabbit Polyclonal Antibody. 인간, 마우스, 랫트 반응성. Western blot, IHC, ICC/IF에 사용 가능. 액상 형태로 제공되며 -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-ASK1 (Thr838) Polyclonal Antibody
Applications and Tested Dilutions
- Western Blot (WB): 1:1,000–1:3,000
- Immunohistochemistry (Paraffin) (IHC-P): 1:50–1:200
- Immunocytochemistry (ICC/IF): 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP3K5 (Accession Q99683), corresponding to amino acid residues around phosphorylated Thr838 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903133 |
Product Specific Information
This antibody detects endogenous levels of ASK1 only when phosphorylated at Thr838 (mouse at Thr845).
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated in response to extracellular stimuli such as cytokines, growth factors, and environmental stress.
ASK1 (also known as MAPKKK5) activates MAPKKs MKK4 and MKK6, which in turn activate JNK and p38 MAP kinases. Activation occurs via TNFR and Fas through TRAF family and Daxx interaction. Overexpression induces apoptosis, while inactive ASK1 inhibits TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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