Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Ultra Violet 563

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) expressed in CD45.2 mouse strains such as C57BL/6, BALB/c, C3H/He, DBA/2, and others. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; use ≤ 1.0 µg/test (100 µL final volume)
• Recommended: Titrate antibody for optimal performance

Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye emitting at 564 nm, optimized for UV (355 nm) lasers. Ensure instrument compatibility.

Staining and Handling Recommendations

• When using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect vial and stained samples from light.
• Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45.2 (leukocyte common antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells. It regulates Src-family kinases, lymphocyte development, and antigen signaling. Isoforms arise from alternative splicing and differ in extracellular domains, influencing membrane localization and cell-type-specific expression. CD45.2 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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