Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience
TLR3(인간 CD283)에 특이적인 단클론 항체로, 면역학 및 병원체 인식 연구에 적합합니다. Flow cytometry 등 다양한 응용에 사용 가능하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 높은 순도(>90%)와 낮은 엔도톡신 수준(<0.001 ng/µg)을 보장합니다.
- 카탈로그번호
- 16-9039-xxx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience
Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 2 µg/test | View 3 publications |
| Neutralization (Neu) | Assay-Dependent | View 1 publication |
| Functional Assay (Functional) | Assay-Dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLR3.7 |
| Conjugate | Functional Grade |
| Available Formats | Unconjugated, Biotin, PE, Request custom conjugation |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866327 |
Product Specific Information
Description:
The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). This receptor family includes at least twelve members characterized by extracellular leucine-rich repeats and cytoplasmic domains homologous to the IL-1 receptor. TLR3 recognizes double-stranded RNA (dsRNA), activating NF-kappaB through MyD88-dependent and -independent pathways, and inducing type I interferons (IFNs). TLR3.7 suppresses poly(I):poly(C)-mediated IFN-beta production by human fibroblasts expressing TLR3.
Applications Reported:
Used in flow cytometric analysis and in vitro inhibition of ligand binding. For optimal flow cytometry detection, PE- or Biotin-conjugated TLR3.7 antibodies are recommended.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines. Recommended usage ≤ 2 µg/test (100 µL final volume). Cell count per test may vary between 10⁵–10⁸ cells. Antibody titration is advised for optimal performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90%, determined by SDS-PAGE.
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10%, determined by HPLC.
Target Information
TLR3 (Toll-like receptor 3, CD283) is a member of the TLR family involved in pathogen recognition and innate immunity activation. These receptors detect pathogen-associated molecular patterns (PAMPs) and mediate cytokine production for immune responses.
TLR3 is highly expressed in placenta and pancreas, mainly in dendritic cells. It recognizes viral dsRNA, activates NF-kappaB, and induces type I interferons. TLR3 signaling utilizes TRIF instead of MyD88, leading to IRF-3 and NF-kB activation. This pathway is crucial for antiviral defense and dendritic cell maturation.
TLR3 has been implicated in recognition of viruses such as Influenza A and West Nile Virus and may facilitate viral entry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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