Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience

Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). This receptor family includes at least twelve members characterized by extracellular leucine-rich repeats and cytoplasmic domains homologous to the IL-1 receptor. TLR3 recognizes double-stranded RNA (dsRNA), activating NF-kappaB through MyD88-dependent and -independent pathways, and inducing type I interferons (IFNs). TLR3.7 suppresses poly(I):poly(C)-mediated IFN-beta production by human fibroblasts expressing TLR3.

Applications Reported:
Used in flow cytometric analysis and in vitro inhibition of ligand binding. For optimal flow cytometry detection, PE- or Biotin-conjugated TLR3.7 antibodies are recommended.

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines. Recommended usage ≤ 2 µg/test (100 µL final volume). Cell count per test may vary between 10⁵–10⁸ cells. Antibody titration is advised for optimal performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

TLR3 (Toll-like receptor 3, CD283) is a member of the TLR family involved in pathogen recognition and innate immunity activation. These receptors detect pathogen-associated molecular patterns (PAMPs) and mediate cytokine production for immune responses.
TLR3 is highly expressed in placenta and pancreas, mainly in dendritic cells. It recognizes viral dsRNA, activates NF-kappaB, and induces type I interferons. TLR3 signaling utilizes TRIF instead of MyD88, leading to IRF-3 and NF-kB activation. This pathway is crucial for antiviral defense and dendritic cell maturation.
TLR3 has been implicated in recognition of viruses such as Influenza A and West Nile Virus and may facilitate viral entry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.