Thermo Fisher Scientific NCAPD2 Polyclonal Antibody
Human NCAPD2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P)에서 검증됨. 항원 친화 크로마토그래피로 정제된 액상 제품이며, PBS 버퍼와 40% glycerol 함유. 세포 분열 관련 condensin complex 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NCAPD2. Recombinant protein control fragment (Product # RP-97627) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2644504 |
Product Specific Information
Immunogen sequence:
DKSVLVCKNA IQLLASFLAN NPFSCKLSDA DLAGPLQKET QKLQEMRAQR RTAAASAVLD PEEEWEAMLP ELKSTLQQLL QLPQGEEEIP EQIA
Highest antigen sequence identity:
- Mouse: 87%
- Rat: 88%
Target Information
Regulatory subunit of the condensin complex, required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain and promote the resolution of double-strand DNA catenanes between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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