Thermo Fisher Scientific XPF Monoclonal Antibody (OTI3H10), TrueMAB
XPF 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 사용 가능. Human, Mouse, Rat, Dog 시료 반응성. Affinity chromatography로 정제된 1 mg/mL 액상 항체로, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H10 |
| Immunogen | Full length human recombinant protein of human ERCC4 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The structure-specific ERCC1/XPF endonuclease complex is involved in the repair of two types of DNA lesions: nucleotide excision repair (NER) for UV-induced lesions and bulky chemical adducts, and recombination repair of interstrand cross-links.
In NER, dual incisions are made on both sides of the DNA damage by two nucleases: XPG cleaves 3′ of the lesion, while the ERCC1/XPF complex makes the 5′ incision.
ERCC1/XPF may also play a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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