Thermo Fisher Scientific UBA2 Monoclonal Antibody (OTI4A3), TrueMAB
UBA2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. HEK293T에서 생산된 인간 UBA2 전장 단백질로 면역화. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A3 |
| Immunogen | Full-length human recombinant protein of human UBA2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting method: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
UBA2 is involved in the covalent attachment of one protein to another, a key posttranslational modification.
Ubiquitin is a well-known protein modifier, and its activation and transfer have been extensively studied.
SUMO (Sentrin), a ubiquitin-like protein, contributes to stabilization and localization of target proteins.
SUMO conjugation requires the coordinated action of SAE1/SAE2 (E1-activating enzyme), Ubc9 (E2-conjugating enzyme), and an E3-like SUMO ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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