
Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PerCP-eFluor 710, eBioscience
마우스 Ly-6G(Gr-1)에 특이적인 단클론 항체로, PerCP-eFluor 710 형광체 결합. Flow Cytometry에 최적화되어 있으며, 0.125 µg/test 사용 권장. 4°C 암소 보관, 광민감성 주의. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573892 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b.
Unlike RB6-8C5, 1A8-Ly6g binds only to Ly-6G on neutrophils and has been used to deplete neutrophils with reported antagonist activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration recommended.
Fluorochrome: PerCP-eFluor® 710
- Excitation: 488 nm (Blue Laser)
- Emission: 710 nm
- Recommended filter: 710/50 bandpass (695/40 acceptable)
- Light sensitive: Protect from light
- Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
- Storage after fixation: Up to 3 days at 4°C in dark
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D)
- Component of myeloid differentiation antigen Gr-1 (with Ly6C)
- Molecular weight: ~21–25 kDa
- Expressed in bone marrow myeloid cells
- Marker for neutrophils, monocytes, granulocytes
- Involved in granulocyte differentiation and maturation
- Suggested role in anti-tumor response and modulation of local immune activity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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