Thermo Fisher Scientific APOBEC3G Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DEHSQDLSGRLR) corresponding to the C-terminus amino acids of APOBEC3G (aa 365-377). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:APOBEC3G,uniProtId:Q9HC16-1,ncbiNodeId:9606,antigenRange:365-377,antigenLength:384,antigenImageFileName:PA5-18701_APOBEC3G_Q9HC16-1_Goat.svg,antigenImageFileNamePDP:PA5-18701_APOBEC3G_Q9HC16-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10984737

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5-a which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.