Thermo Fisher Scientific APOBEC3G Polyclonal Antibody
APOBEC3G 단백질을 인식하는 Thermo Fisher의 염소 폴리클로날 항체로, Western blot 및 IHC(P) 검증 완료. 합성 펩타이드 면역원 기반으로 제조되었으며, 0.5 mg/mL 농도의 액상 형태. 인간 시료 반응성, 연구용 한정.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (DEHSQDLSGRLR) corresponding to the C-terminus amino acids of APOBEC3G (aa 365–377) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984737 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
The Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like (APOBEC) 3 family is a group of cytidine deaminases that act on monomeric nucleoside and nucleotide substrates. APOBEC3 plays a crucial role in cellular defense against retroviral infection. Isoforms such as APOBEC3G and APOBEC3F can be incorporated into HIV-1 virions, inducing hypermutation in viral DNA and destabilizing the viral genome. The HIV-1 Vif protein counteracts this defense mechanism by inducing ubiquitination and degradation of APOBEC3G. A single amino acid substitution (D128K) can block APOBEC3G depletion without affecting its antiviral activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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